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yabJ [2020-06-19 11:45:29]
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yabJ [2020-06-19 11:45:29]

2-iminobutanoate/2-iminopropanoate deaminase, detoxification of reactive intermediates generated in isoleucine biosynthesis
locus
BSU_00480
pI
5.13
mw
13.51 kDa
protein length
125 aa Sequence Blast
gene length
378 bp Sequence Blast
function
deamination of reactive intermediates generated in isoleucine biosynthesis
product
2-iminobutanoate/2-iminopropanoate deaminase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]

    • Gene

    Coordinates
    55,295 55,672

    The protein

    Catalyzed reaction/ biological activity

  • release of ammonia from reactive enamine/imine intermediates of the pyridoxal 5'-phosphate-dependent threonine dehydratase ([protein|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|IlvA]) [pubmed|22094463]
  • 2-iminobutanoate + H2O = 2-oxobutanoate + NH3 [pubmed|22094463]
  • 2-iminopropanoate + H2O = pyruvate + NH3 [pubmed|22094463]

    • Protein family

  • RutC family (single member, according to UniProt)

    • Structure

  • [PDB|1QD9] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=11484 NCBI] [Pubmed|10557275]
  • [PDB|5Y6U]

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • additional information

  • was originally thought to be required for activity of [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR], but this is due to the orientation of the marker gene downstream of [gene|search|purR ]in the [gene|search|yabJ ]mutant strain used in [pubmed|10368157]

    • Expression and Regulation

    Operons

    genes
    [gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, [Pubmed|7638212], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]

    • regulation

  • negative autoregulation [Pubmed|7638212]
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    Biological materials

    Mutant

  • MGNA-B911 (yabJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1910 NBRP B. subtilis, Japan]
  • BKE00480 ([gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT, downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
  • BKK00480 ([gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT, downstream forward: _UP4_TAATAAGAAAAGTGATTCTG

    • References

  • 10368157,7638212,7638212,10557275,22094463,31272833